Frozen Tissue Array

Why choose frozen tissue over paraffin embedded tissue?
As the frozen tissue products are more expensive than the traditional paraffin embedded tissue products, why so many researchers still choose to use frozen tissue products?

Intensity of IHC comparison between frozen and paraffin tissue sections

FIG. 4. Immunohistochemical localization of CART peptide in the hypothalamus (B–F) and pituitary gland (I and J). AHN, Anterior hypothalamic nucleus; ME, median eminence; V3, third ventricle. Negative controls are shown in A, G, and H. Neuronal cells and fibers in the PVN of a paraffin section are shown at higher magnification in C, whereas comparable regions in a frozen section are shown in E. Similarly, neurons in the ARH are shown in both paraffin and frozen sections (D and F, respectively). Whereas cellular staining was comparable between paraffin and frozen sections, the staining of fibers was less intense in paraffin section. The median eminence, shown in D and F, showed dense fibrous staining in its external layer. In the anterior pituitary, CART-positive cells were found mainly in peripheral regions, with some of these cells located around blood vessels (I). In the posterior pituitary, stained varicose fibers were observed throughout the lobe, whereas CART-positive cell bodies were not found (J).

Kuriyama, G., S. Takekoshi, K. Tojo, Y. Nakai, M. J. Kuhar, and R. Y. Osamura. 2004. Cocaine- and amphetamine-regulated transcript peptide in the rat anterior pituitary gland is localized in gonadotrophs and suppresses prolactin secretion. Endocrinology 145:2542-50.

Intensity of ISH comparison between frozen and paraffin tissue sections

Methods: Method for Creating a Frozen Tissue Array
United States Patent: US00690009B2